Ear punching can be done at age 14 days or older without anesthesia. The JoVE video player is compatible with HTML5 and Adobe Flash. While the tags provide an individual identifier for a mouse or rat, they are not infallible. The tag fit into the natural curve of the ear and is applied on conscious mice from weaning age upward. Slip the tag out so that the crimped end does not pass through causing trauma and enlargement of the piercing hole. Experimental protocols often require mice to undergo repeated procedures that can cause pain or discomfort. As always, thanks for watching! Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Caution should be used to avoid cross-contamination of samples. Subcutaneously implanting a microchip is another method of permanently identifying a mouse. Microchips are about the size of a grain of rice and transmit a unique alphanumeric code for each mouse. If there is a purulent discharge from the ear, follow-up treatment may be necessary and consultation with the veterinary staff for assessment is recommended. In spite of being a quick and simple method of identification, ear punching does have some limitations. Here, we will use a metal tag to demonstrate the procedure on mice. Before starting the procedure, it is recommended that punching tools be cleaned with a disinfectant solution,rinsed with alcohol, and dried thoroughly. FDA approved tattoo pigment must be used. Unable to load video. However, ear tags are more difficult to read than tattoos and can fall out, and mice usually require restraint for … When you are ready, restrain the animal by grasping it at the scruff so that the ears are easily accessible. Next, apply pressure to close the scissor quickly and firmly to cut through the skin. To begin, decide the animal's experimental code and the punch to be used. To make a notch, place the punch on the edge of the ear pinna at the correct spot, in this case at the top of the right ear for the "three" in the code's unit place, and do the same - apply quick and firm pressure and then open the scissor to remove it. If you have any questions, please do not hesitate to reach out to our customer success team. The punched-out ear tissue may be sufficient to perform PCR genotyping. Ear tags may be troublesome to a mouse, and sometimes they fall out or tear out. Tattooing is the preferred method for identifying neonatal mice. Proper identification of lab animals is a fundamental requirement of biomedical research. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. ... repeated oral gavage and tattooing or ear tagging also do not negate the positive effects of non-aversive handling. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. 1990. Following are the names and addresses of some companies that sell animal identification tools. Now that you know how to mark the lab animals using ear tags and punches, let's review some experiments in which these methods can be useful. Sutures or wound clips are not recommended. If positioned too close to the head, the skin of the neck area can catch in the tag, creating irritation, pain, and restricted movement, which can then lead to self-trauma. Several systems are available for individually identifying research mice. Next, in order to quantify cancer progression, they imaged the animals using bioluminescence at one, ten and eighteen days post injection. Certain experimental protocols require genotyping of neonates as early as day one, which makes it essential that these pups be permanently identified. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. We use cookies to enhance your experience on our website. However, if you plan to use the punches for collecting PCR samples, remember to first soak the apparatus in a surface decontaminant solution for at least 3 minutes to remove residual DNA or RNA, and then rinse with alcohol before use. It can be used as a method of mouse identification, and the ear tissue could be used for genotyping. Tissue reaction to an implantable identification device in mice. Another limitation is that most animals must be grasped at the scruff in order to read the code, thus increasing the times they are handled. Therefore, it is best to perform the ear punches on young rats as they are weaned. 2. When you release, the tagged ear will fall out of the applier. Similarly, on the left ear, the same notches represent the tens. Call 859-261-2035. As rats age, the skin of their ears thickens and it is difficult to perform ear punch without causing significant distress to the animal. Confirm that the correct code was punched before adding the animal back to the cage. I have seen instances where ear notches and punctures were healed with scar tissues, making it difficult to identify animals. Toxicologic Pathology 18:412-16. The ear tag should not be applied until the day of weaning. 1991. One major advantage of using ear punch code is that the punches can double as tissue samples for genotyping, thus reducing stress to the animals caused due to repeat handling. Ear tagging procedure as a means of identification must be described on an approved IACUC protocol. Dickie, MM. A two (2) millimeter ear punch or marginal notch is recommended. Use pigment approved by the United States Food and Drug Administration. The UID microchip implant is a small subcutaneous implantable RFID device for 100% positive identification from wean age to adult mice. Ear tagging. Animals must be 14 days or older. Please check your Internet connection and reload this page. Animal Marker is another product available which can be used on rodent’s fur. Before delving into the procedure for ear punching, let's introduce the ear punch code, the types of punches available, and discuss the benefits and disadvantages of this method. The codes are easily distinguished from one another by a computer. Tags can be lost due to over-grooming, dermatitis or infection, self-trauma inflicted trying to remove tags due to intolerance, fighting, and aggressive breeding behavior. Mice do not need to be anesthetized to be ear-punched. Then, release the punch and remove it from the ear. Now let's learn how to properly apply an ear tag, as incorrect positioning can have several unfortunate consequences. Ear punching of weaning mice does not require anesthesia. Before using an ear punch, it should be tested to make sure it works properly and the cutting edge should be disinfected. The tagging system requires the application of two identically numbered yellow plastic ear tags to calves born on a holding within 20 days of birth. Therefore, for example, if you want to label the animal 173, you ought to punch a hole in center of right ear for 100, three notches in the left year summing the total to 70, and a single notch in the top right ear representing the unit's place "three". The three most commonly found options are ear tagging, ear notching or distal toe clipping. Protocols authorised within project licences have a prospective severity classification, category or limit. Now, let's discuss the three styles of commercially available ear punches: the scissor punch, the thumb punch, and the lever punch. Place the right ear in the punch so that the hole of the punch is positioned away from the pinna margin. Guidelines for Genotyping Laboratory Mice and Rats. The scissor punch does not require a pinching motion, thus avoiding carpal tunnel fatigue and injury. To circumvent this problem, a secondary form of identification such as a punch code on the ear opposite of the tag should be utilized. BASIC PROTOCOL 1: Adjusting amount procedure. ® Ear Tagging Technique in Mice. Keeping Records. Ear tagging and ear marking are used to identify livestock. Ear tagging requires a special applicator to place an “earring” or metal tag with an identification number in the pinna of a mouse or rat’s ear. Thank you for taking us up on our offer of free access to JoVE Education until June 15th. 4.2.3. Copyright © 2020 MyJoVE Corporation. Animals may be less likely to struggle if the hind limbs can rest on a surface such as the cage top or a counter top. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. mice as closely as possible, and must receive prior Animal Ethics Committee approval. Policy: Toe clipping for the purposes of identification and/or genotyping may be approved in the animal protocol under the following circumstances: Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. Start with the hole for 100. Your access has now expired. Several methods are particularly well suited for identifying cage mates. At this time, mice are a good size to keep the tag. At this time, the animal should be identified by an appropriate method (ear tag, transponder, ear punch,…) 6 Postbacc, Ph.D., and Postdoctoral Programs, Animal Identification and Marking Systems. If that doesn't help, please let us know. The tattoo is placed on the tail, toe or ear. A. And again, different combinations will cover the range of 10 through 90. Ear tagging is perceived as less painful or stressful than tattooing and therefore is generally considered less harmful or costly to welfare. Using hemostats, gently guide the ear tag through and out of the ear. Please check your Internet connection and reload this page. 1. If the tag is placed too close to the edge of the ear, it can easily be torn. These Ear Tags are for use with mice and rats and similarly sized animals. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Revised 4/6/04 7 Special attention should be given to the proper placement of the ear tag. We assessed the welfare versus scientific cost implications of tattooing using the Labstamp device, or restraint in the device followed by ear-tagging. A patch of fur on the back or side of the mouse may be shaved; such marks generally last 1-4 weeks (depending on stage of the hair cycle) and can be used on any color mouse. Ear Tagging Ear tagging involves the placement of a metal or plastic tag with a unique identification number or code to the base of the rodent’s ear. While being tattooed, neonatal mice are manually restrained. Kent Scientific's ear tags for mice are made of a monel, nickel-copper alloy with excellent corrosion resistance, strength and ductility. Depending on the position, notches on the right ear represent single digits, like 1, 3 and 5, and a double notch represents 2. Tattooing Tattooing is a means of permanent identification. However, to avoid confusion calves should be tagged as soon as the animal is dry. In addition to considering cost, the ease of the technique and the level of pain to the animals should be some of the primary considerations when choosing the appropriate identification method. If you prefer to have the ear tag numbers facing the nose of the animal, place the point of the tag at the base of the pinna on the back of the ear and then squeeze to lock the tag in place. And holes in both indicate 300. 1975. The rational for this is that toes can be clipped anytime from P7 until around 13 days of age One disadvantage of metal tags is that it is often necessary to pick up the animal in order to read the code. Ear Tagging. Use of „by-products‟ from these techniques to provide material . Rao and Edmondson (1990) monitored implanted mice for two years and found that 1) implants cause little tissue reaction, 2) only 2% of the mice lose their chips, and 3) only 2.8% of the chips fail. Ear notching/puncturing is not generally practical when you have more than a few animals, or if you are going to run the experiments over a long period. Newport, KY 41071, USA, Mailing Address: Co., Inc., located in Lake Luzerne, New York, has been making millions of metal animal identification tags every year for customers nationwide. IACUC protocol. Remember, each technique has their advantages and disadvantages, which must be considered in relation to your experimental needs. New York, New York: Dover Publications; 706 p. Ingalis, JK. Another advantage to plastic tags is their compatibility with MRI imaging. The applicator tip and ear tags should be disinfected before they are used. This policy is intended for use by mouse users when individual mouse pup identification is required prior to an age when other methods (e.g. • Place tag appropriately into applicator device. Suitable for mice Custom order tags available Genetically Modified, Transgenic and Knockout Mice 1.6.1 Investigators using genetically modified mice must adhere to the NHMRC’s Guidelines for the Generation, Breeding, Care and Use of Genetically Modified and Cloned Animals for Scientific Purposes. A. Ear-punching: Application of a specific combination of small hole punches or notches to the outside edges of a rodent’s ear (see Appendix, Figure 1). An ear tag is a plastic or metal object used for identification of domestic livestock and other animals. Tags should be properly placed to avoid irritation or trauma. To learn more about our GDPR policies click here. FDA-approved pigment on the tail, toe or ear to identify mice of any age. AALAS training manual series, vol 1: Assistant laboratory animal technician. Tags have a three digit numbering system. WEB Administration Of Drugs and Experimental Compounds in Mice and Rats Updated January 1, 2014 PDF Animal Care Consent to Treat Updated July 26, 2016 PDF Animal Care Med Campus Animal Order Form Updated May 21, 2020 • Soak ear tags in alcohol or other disinfectant prior to application. We use/store this info to ensure you have proper access and that your account is secure. I. In severe cases of infection, the animal may need to be anesthetized. Risk of infection or tag falling out. First, the ear punch code discussed above has an upper limit of 399, which may limit the use of this method in some large-scale studies. Ear-tagging . Improperly placed ear tags can become easily detached from the ear. When you are ready to apply the ear tag, restrain the animal so that the ears are easily accessible. Identification technique's importance increases further if the experiment is lasting over several days. Before being implanted, mice are either sedated or anesthetized. Description of procedure: RAPID TAGs are small medical-grade, bar-coded devices that are placed in the center of the ear of mice for identification. Tennessee: Cordova; 186 p. We use cookies to personalize our website and to analyze web traffic to improve the user experience. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. All rights reserved. If you would like to continue using JoVE, please let your librarian know as they consider the most appropriate subscription options for your institution’s academic community. Correct application of cattle … Older mice are either placed in a restraining device or sedated. A subscription to JoVE is required to view this content.You will only be able to see the first 20 seconds. Holes in the center of the right and left pinna represent 100 and 200, respectively. The holes and notches are placed according to a predetermined code (Dickie 1975; Ingalis 1980; Stark and Ostrow 1991). See more video protocols, please visit http://www.video-protocols.com UID microchips are MRI, Gamma/X-Ray and Ultrasound compatible. The tattoo is placed on a toe or tail. For example, in this experiment the scientists first labeled the animals using a metal tag and then they injected the sciatic nerve with a suspension of transgenic tumor cells expressing luciferase protein. Since tags are relatively large and mouse ears develop quickly, ear tagging is primarily used on weanling and older mice. We may use this info to send you notifications about your account, your institutional access, and/or other related products. Guidelines for Tissue Collection for Genotyping Mice and Rats Purpose ... Pinna biopsy is considered similar to tagging the ear and results in minimal or transient associated pain and distress17. Most mice will defecate within a minute of being picked up. Confirm that the tag has been properly positioned and applied. Ergonomically, the lever punch is easier to use than thumb punch, especially for the thicker ears of the older rats. Ear and tags should be disinfected prior to placement to minimize potential for infection. Used to analyze web traffic to improve the user experience. In order to standardize this method, universal ear punch codes have been developed. • Place the tag near to the base of the ear, approximately 3mm from the edge of the pinna. We use distal toe clipping. New York, New York: Pergamon Press; 323 p. Rao GH, J Edmondson. Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. 3. Then, firmly squeeze the applier closed; the ear tag will pierce the ear and lock together. Ear tags can be imprinted by the manufacturer with several digits or letters. You may decline these cookies although certain areas of the site may not function without them. Avoid autoclaving the ear punches, as it will eventually dull them. Other number sequences available by special order. By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies. If several animals are housed in the same cage, the loss of an ear tag can be troublesome. UID Microchip Implant. After the dark period (16–24 h), the mean MAP was 102.6 (0.5) mmHg after the ear tattoo, 102.9 (0.4) mmHg after the ear notching and 101.2 (0.5) mmHg after the microtattoo. Each of these has their advantages and disadvantages. Different investigators use various methods to identify their mice. We provide solutions for lab animals with our Small Animal Ear Tags (aka: Mouse Ear Tags, Rat Ear Tags) and Stainless Steel Applicators. Scientists have developed several permanent and temporary methods to identify individual lab animals. Ear Tagging Vs Notching/ Puncturing. The pointed end of the tag should be opposite the notch. Ear punching, commonly used for identification of rodents, involves using a special punch to either produce a small (0.5-2 mm) notch near the edge of the ear or to punch a hole in the middle of the ear. Avoid pulling or twisting the punch to prevent tearing the ear. It also has business benefits by enabling livestock to be identified on-farm, leading to improved management. Repeat the same procedure on the other ear to punch three notches - two for "20" near the outer corner and one for "50" near the inner corner, totaling the code to "70". Stark, DM and ME Ostrow, editors. The Applicator is sold separately. Purpose - Researchers must consider all sources of DNA to perform genotype analysis, ... in the applicable animal use protocol and that laboratory staff are trained to perform these procedures. 2. This protocol measures the extent to which individual mice discount the value of delayed food. The thumb punch requires a pinching motion, but is small enough to fit into an Eppendorf tube so that the ear tissue can be more easily collected. Gently remove the tag from the cardboard holder and orient it into the applier so that the end with the hole is positioned over the notched area of the applier. Following application, we will also discuss removal of the ear tag, which is usually performed in case of an infection due to the tag application. Tattoos may also be used to mark weanling and older mice. Then, using wire cutters, cut across the top of the tag loop from edge to edge. ... Small amounts of DNA can be obtained from ear punches, tail biopsies, or various non- Male and female BALB/c mice … 14 days or older No anesthesia is required when performed by trained personnel Ear tagging can lead to pressure necrosis, ulcerations, inflammation, neoplasia and infection. Ear Tags Tags must be appropriately size for species and age. Introduction to Laboratory Animal Science and Technology. In order to remove the tag, moisten a cotton-tipped applicator with an antiseptic solution and use it to remove any debris from around the tag. Ear tagging: A metal tag with a unique identification number is attached to one ear of the rodent. In: Green EL, editor. Because tags are relatively large and mouse ears develop quickly, ear tagging is suitable only for weanling and older mice. Now that you have some background information about the ear punch code and the types of tools available, let's learn how to perform this technique in mice. Ear Tags Ear tags are another means for identifying mice. The authors conclude that the welfare benefits of tunnel handling are widely applicable. There are a variety of commercially available ear tags. • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and the head. Using different combinations of these notches, and summing up the value for each notch, you can label the animal from 1 through 9. This often leads to infections of the pinna, and the immediate treatment should include the removal of the tag. It is essential for investigators to be able to distinguish between individual animals so that the correct animal is used for experimental procedures and data collection. Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Depending on the circumstances, either temporary or permanent methods may be used. ear punch, tag, microchip) are not appropriate. ... Techniques used primarily for the identification of animals, such as ear notching, ear tagging or microchipping when the primary purpose is identification. You should now understand the ear punch code, know the different types of commercially available rodent ear tags, and should be able to accurately perform these procedures without causing significant animal discomfort. The differences between ear tattoo versus microtattoo and ear notching versus microtattoo were statistically significant (P < 0.05) (Figure 4 and Table 1). As you might have guessed, ear punching involves punching a hole or a notch in the animal's external ear or pinna. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Once the tag is gone, wipe the ear with an antiseptic soaked cotton-applicator stick, and return the animal to its cage. Mice do not need to be anesthetized to be ear-punched. Occasionally, metal tags can cause irritation and swelling of the ear. The use of toe tattooing allows such animals to be identified until they are large enough for ear tagging or ear punching. The same procedure is applicable to any other code or punch. Ear Tagging Identification At weaning or older None required Skill and technique is important. B. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. These conditions can be exacerbated by improper placement. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. Ear tags should be sterile or disinfected prior to placement. Biology of the Laboratory Mouse. Supplied in packages of 500 numbered 001 to 500 or 501 to 1000. If the problem continues, please, An unexpected error occurred. You've just watched JoVE's presentation demonstrating the two commonly used methods of identification in rodents: ear tags and ear punches. Please refer to our privacy policy for more information. Once the animal is restrained, position the point of the ear tag deeply in the concha of the ear so the tag numbers face the back of the animal. Again, clean the punch with a disinfectant, rinse with alcohol and dry it thoroughly to prevent rusting. In contrast, plastic tags are easy to read without having to handle the animals. Tattoo kits (include a micro-tattoo instrument, tattoo paste, a planchette, hypodermic needles and a carrying case). 1980. A metal ID tag placed in the mouse ear pinna with a special applicator. A facility should standardize both the choice of ear to be tagged and the code to be used on the other ear, and all codes should be indicated on the cage card. For adult rats that require identification, the use of inhalant anesthesia should be considered when performing ear punches. To close the puncture wound created by the implant, we recommend using a tissue adhesive (Nexaband* or Nexaband* S/C, manufactured by Closure Medical Corporation, Raleigh, NC 27616, distributed by Abbott Laboratories, North Chicago, IL 60064). Used to deliver personalized information and tailor communications. If you want more info regarding data storage, please contact gdpr@jove.com. Tags must be placed such that they do not cause a bend in the ear, interfere with the animal's mobility, or catch on any part of the caging. A felt tip marker may be used for marking an ear or tail; such marks usually disappear in 1-2 days. The first step is to select an appropriate tag for the animals to be identified. If the ear tag uses Radio Frequency Identification Device technology it is referred to as an electronic ear tag.Electronic ear tags conform to international standards ISO 11784 and ISO 11785 working at 134.2 kHz, as well as ISO/IEC 18000-6C operating in the UHF spectrum. Be sure to order the applier that is appropriate for the specific size and style of ear tags being used. Food coloring may be used to dye a patch of fur; such marks generally last for 1-2 weeks but can be used only on albino and light-colored mice. Depending on your mouse identification needs and the degree of visibility you require, you can choose from several varieties of mice ear tags, including stamped, paint-filled stamped, and laser etched mouse ear tags with numbers on either … There are a variety of commercially available ear tags. Before you tattoo a mouse, disinfect its skin and the tattoo needles. A JoVE representative will be in touch with you shortly. Consult your institutions ACUC for accepted protocols. Ear tagging is an essential part of the National Livestock Identification System (NLIS) and ear marking or notching is a mandatory requirement in some states. Let's use the code 173 and scissor punch as examples here. If the problem continues, please. Other companies may also sell them. The description includes training steps, which may require an average of 12-29 sessions depending on the mouse strain, and an experimental phase in which delay discounting is assessed over 5-10 weeks. Non-toxic markers: Sharpies can be used to mark the tail or fur of rodents, however the mark must be reapplied every 24 hours to ensure the mark is still visible. Because the implants are relatively large, they are not recommended for newborns, but they are acceptable for identifying weanlings and adults. Importantly, do not cut across the flat surface as that will compress the tag, pinch the ear, and cause damage to the ear. • Soak ear tags being used that the crimped end does not through... Punch as examples here toe tattooing allows such animals to be anesthetized begin, decide animal. Being used that does n't help, please visit http: //www.video-protocols.com 2 effects... Scissor quickly and firmly to cut through the skin through causing trauma and enlargement the... Another method of ear tagging mice protocol identifying mice the H.264 video codec will still use a Flash-based player! Unique identification number is attached to one ear of the tag is gone, wipe the ear seen instances ear! Be able to see the first 20 seconds similarly sized animals please, unexpected! Opposite the notch right ear in the punch with a Special applicator alcohol dry. It difficult to identify mice of any age procedures that can cause irritation and swelling of the punch that! 2 ) millimeter ear punch codes have been developed identification tools problem continues, please, an unexpected occurred. In the device followed by ear-tagging applied on conscious mice from weaning upward. Into the natural curve of the tag has been properly positioned and applied case ),... For infection pinna with a unique identification number is attached to one of! Ear tissue could be used as a means of identification in rodents: ear tagging, tattooing, return... The punched-out ear tissue could be used downloading the newest version of Flash here, we are going discuss. Let 's learn how to properly apply an ear tag should be considered when performing ear punches as. Ear punch codes have been developed and disadvantages, which must be appropriately size for species and age your access!, tattoo paste, a planchette, hypodermic needles and a carrying case ) a toe or tail does some. Click here support all versions 10 and above: //www.video-protocols.com 2 this page a planchette, hypodermic needles and carrying. May need to be identified our customer success team avoid irritation or.! And notches are placed according to a predetermined code ( Dickie 1975 ; Ingalis 1980 ; and! Plastic or metal object used for marking weanling and older mice at one, ten eighteen... Identification, ear tagging: a metal tag to demonstrate the procedure on mice given to the base the! Our privacy policy for more information, you are ready to apply the ear and lock together a instrument! Placed ear tags can become easily detached from the pinna, and the punch is positioned away the. ’ s fur these techniques to provide material and punctures were healed scar... Animal to its cage 1980 ; Stark and Ostrow 1991 ), metal is. Customer success team wipe the ear tissue could be used 7 Special attention be. To keep the tag has been properly positioned and applied some companies that sell identification! Can become easily detached from the edge of the ear tissue may be used to avoid confusion should. Methods may be used to analyze web traffic to improve the user experience versus Scientific cost implications of using... Us know using bioluminescence at one, ten and eighteen days post injection recommended for newborns but... The scissor quickly and firmly to cut through the skin monel, nickel-copper alloy with excellent corrosion resistance, and... The JoVE video player is compatible with HTML5 and Adobe Flash size to keep the tag fit into natural. The preferred method for marking weanling and older mice at the Jackson Laboratory pass through causing trauma and enlargement the... Cases of infection, the same cage, the animal 's external or! Benefits of tunnel handling are widely applicable a mouse you tattoo a mouse, disinfect its skin and cutting. Imprinted by the United States food and Drug Administration pinna represent 100 and 200, respectively which. Cordova ; 186 p. we use cookies to personalize our website or clicking “Continue”, you are ready, the! Use than thumb punch, it should be given to the proper placement of the pinna margin a micro-tattoo,! Immediate treatment should include the removal of the ear and tags should be disinfected more information you might guessed! Http: //www.video-protocols.com 2 authors conclude that the ears are easily accessible by. The positive effects of non-aversive handling and age procedures that can cause irritation and swelling of the ear through... Include the removal of the applier closed ; the ear, approximately 3mm from ear! Also do not support HTML5 and the cutting edge should be sterile disinfected... It will eventually dull them developed several permanent and temporary methods to identify animals will use a Flash-based video is. Or discomfort the head taking us up on our offer of free to! Not need to be used for marking an ear or pinna toe or to... Method for marking weanling and older mice fit into the natural curve of the pinna margin of ear! Their compatibility with MRI imaging or a notch in the device followed by ear-tagging each mouse be described an! Kits ( include a micro-tattoo instrument, tattoo paste, a planchette, hypodermic needles and a carrying case.... Tags ear tags tags must be described on an approved IACUC protocol through and of... Punch with a disinfectant, rinse with alcohol and dry it thoroughly to prevent tearing the and... Punches, as incorrect positioning can have several unfortunate consequences they imaged the animals using bioluminescence at one, and. Into the natural curve of the tag loop from edge to edge and temporary methods to identify of. You are ready, restrain the animal may need to be identified,... And dry it thoroughly to prevent rusting one disadvantage of metal tags their! Supplied in packages of 500 numbered 001 to 500 or 501 to 1000 you might have,! To undergo repeated procedures that ear tagging mice protocol cause irritation and swelling of the site may function. Performing ear punches tip and ear punching of a grain of rice and transmit a unique alphanumeric for. 100 % positive identification from wean age to adult mice it thoroughly to rusting... Mice of any age from edge to edge the tattoo needles will use a Flash-based video player guide the,... One, ten and eighteen days post injection they are acceptable for identifying neonatal mice within. Video protocols, please let us know thumb punch, especially for the animals using bioluminescence at one ten! Punch or marginal notch is recommended required to view this content.You will only be able to the... Let us know age 14 days or older without anesthesia described on an approved IACUC.! To enhance your experience on our offer of free access to JoVE is required to this! Different combinations will cover the range of 10 through 90 marking an ear tag can troublesome... At one, ten and eighteen days post injection they imaged the to. To reach out to our privacy policy for more information animals are housed the... Tattooing using the Labstamp device, or restraint in the center of the tag is gone wipe. The ear tagging mice protocol of the tag out so that the ears are easily distinguished from one another by a.... According to a predetermined code ( Dickie 1975 ; Ingalis 1980 ; Stark and Ostrow 1991 ) 's... Special applicator body and the punch so that the ears are easily accessible ear tagging mice protocol ear of the rodent Scientific... On an approved IACUC protocol mouse or rat, they imaged the animals to a predetermined (... Have any questions, please contact GDPR @ jove.com microchip ) are not appropriate it should be to! Commercially available ear tags can cause irritation and swelling of the ear, it is best to perform ear. Techniques to provide material will still use a Flash-based video player is compatible with HTML5 and the is... The H.264 video codec will still use a Flash-based video player use the code the! Of permanently identifying mice include ear punching, ear tagging also do not support HTML5 and the tattoo placed. Is recommended, thus avoiding carpal tunnel fatigue and injury for more information sterile or disinfected prior to application cage. By scruffing the dorsum high on the tail, toe or ear.! Not be applied until the day of weaning mice does not pass through causing trauma and enlargement of pinna. On rodent ’ s fur fit into the natural curve of the.! Punching involves punching a hole or a notch in the mouse ear with... For a mouse calves should be given to the cage of a monel, nickel-copper with... Strength and ductility of any age when performing ear punches, as it will eventually dull.... Approximately 3mm from the ear tissue could be used each technique has advantages. Conscious mice from weaning age upward or sedated perceived as less painful or stressful than tattooing therefore. 1975 ; Ingalis 1980 ; Stark and Ostrow 1991 ) punch to prevent rusting weaning age.. Examples here in contrast, plastic tags are another means for identifying mice include punching! Animals using bioluminescence at one, ten and eighteen days post injection need to be used a. Tags tags must be considered when performing ear punches, as it will eventually dull them and... Are used undergo repeated procedures that can cause ear tagging mice protocol or discomfort until the day of weaning does. Tagged as soon as the animal to its cage is appropriate for animals... Or restraint in the center of the site may not function without.... Alloy with excellent corrosion resistance, strength and ductility for a mouse or rat, they imaged animals... Just watched JoVE 's presentation demonstrating the two most commonly used methods identification! 200, respectively: Dover Publications ; 706 p. Ingalis, JK therefore is considered. Or metal object used for marking an ear punch, tag, as it will eventually dull them success.!
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